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Gibco™ DMEM, high glucose

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Overview
17.64 GBP valid until 2025-12-31
Use promo code "24273" to get your promotional price.

Additional Details:
Additional Details: Weight: 0.59000kg



Disclaimers: For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Product Code. 10741574

Quantity Price
1 £ 21.66 / 500mL
In stock, ready to ship: 3573
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  • Description and Specification
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Description and Specification

Specification

Cell Line HeLa, 293, Cos-7, and PC-12
Cell Type Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification Animal Origin-free
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)
Serum Level Standard Serum Supplementation
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Glutamine,Phenol Red
With Additives High Glucose,Glutamine,Phenol Red
With Additives High Glucose,Glutamine,Phenol Red
Without Additives No HEPES,No Sodium Pyruvate
Without Additives No HEPES,No Sodium Pyruvate
For Use With (Application) Mammalian Cell Culture
Glucose Concentration 4500 mg/L
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco
Quantity 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

With: High Glucose, L-glutamine, Phenol Red

Without: Sodium Pyruvate, HEPES

The complete formulation is available.

Using DMEM

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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