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Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Overview
489.00 GBP valid until 2025-06-30
Use promo code "24108" to get your promotional price.

Code: 88

Additional Details:
Additional Details: Weight: 0.97980kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 11813923

Quantity Price
1 £ 978.0 / 1000 reactions
Estimated Shipment
20-06-2025
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Description and Specification

Specification

Content And Storage Contains:
• 10 x 1.25 mL Maxima Probe/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer with ROX passive reference dye; sufficient for 1000 x 25 μL reactions.
• 10 x 1.25 mL Nuclease-Free Water

Store at -20°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase Taq DNA Polymerase
Reaction Speed Standard
For Use With (Equipment) 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Product Line Maxima
Product Type qPCR Master Mix
Quantity 1,000 reactions
Sufficient For 1000 Reactions at 25 μL
For Use With (Application) Gene Expression
Concentration 2X
No. of Reactions 1,000 Reactions
Sample Type DNA, dsDNA
Volume 10 x 1.25 mL

The master mix includes Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. It is supplemented with ROX passive reference dye. Only template and primers need to be added.Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carryover contamination control using uracil DNA glycosylase (UDG). ROX serves as a passive reference dye. The use of Maxima Probe/ROX qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates. The master mix can be used with most real-time thermal cyclers, including instruments from Applied Biosystems, Eppendorf, Corbett, Bio-Rad and Roche.
  • Sensitivity—detects low copy number targets
  • Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Wide linear range—accurate quantification across 9 orders of magnitude
  • Universal—can be used with sequence-specific probes on most real-time thermal cyclers
  • Reproducibility and convenience—ready-to-use 2X master mix minimizes pipetting error and reduces set-up time

Recommended for:

Real-time PCR using sequence-specific probes; Real-time RT-PCR using sequence-specific probes.

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