Thermo Scientific™ Maxima Probe/ROX qPCR Master Mix (2X)

Descrizione

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

• Sensitivity—detects low copy number targets
• Specificity—Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Wide linear range—accurate quantification across 9 orders of magnitude
• Universal—can be used with sequence-specific probes on most real-time thermal cyclers
• Reproducibility and convenience—ready-to-use 2X master mix minimizes pipetting error and reduces set-up time

Recommended for:

Real-time PCR using sequence-specific probes; Real-time RT-PCR using sequence-specific probes.