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Cytiva Single Cell GenomiPhi™ DNA Amplification Kit

Amplify whole genome DNA from as little as a single cell. Cytiva Lifescience™ illustra™ Single Cell GenomiPhi™ DNA Amplification Kit combines unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.

Overview
Code: NEW

Additional Details:
Additional Details: CAS Number: 25322-68-3 Weight: 0.54000kg Transport: UN number : 1814 Chem class : 8 Pack group : II



Product Code. 15301652

Quantity Price
1 £ 401.0 / 25 reactions
Estimated Shipment
19-05-2025
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Description and Specification

Specification

Content And Storage Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
Description Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
Final Product Type illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
For Use With (Application) Genomic DNA Amplification
Includes Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
No. of Reactions 25
Product Type illustra Single Cell GenomiPhi DNA Amplification Kit
Size >10kb
Test Time 2 to 4 hours

  • For whole genome amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
  • Reaction time: 2 to 4 hours
  • Product average size: >10kb
Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


Safety and Handling

[Danger]

[{code=SKIN CORR. 1, sequence=1, description=Skin corrosion/irritation Category 1}, {code=SKIN CORR. 1B, sequence=1, description=Skin corrosion/irritation Category 1 B}]

[{code=H314, sequence=1, description=Causes severe skin burns and eye damage.}]

[{code=P260, sequence=51, description=Do not breathe dust/fume/gas/mist/vapours/spray.}, {code=P301+P312, sequence=52, description=IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.}, {code=P303+P361+P353, sequence=53, description=IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.}, {code=P305+P351+P338, sequence=54, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}, {code=P405, sequence=55, description=Store locked up.}, {code=P501b, sequence=56, description=Dispose of contents/container in accordance with local/regional/national/international regulations.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : potassium hydroxide}]

[{code=GHS05, sequence=1, description=CORROSION}]


Signal Word
  • Danger

Hazard Category
  • Skin corrosion/irritation Category 1
  • Skin corrosion/irritation Category 1 B

Hazard Statement
  • H314- Causes severe skin burns and eye damage.

Precautionary Statement
  • P260- Do not breathe dust/fume/gas/mist/vapours/spray.
  • P301+P312- IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.
  • P303+P361+P353- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P405- Store locked up.
  • P501b- Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental Information
  • MIXTURE LIST- Contains : potassium hydroxide