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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Overview
79.65 GBP valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Code: 88

Additional Details:
Additional Details: Weight: 0.45360kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes:

Phusion High-Fidelity DNA Polymerase

  • Phusion DNA Polymerase 2 U/μL
  • 5X Phusion HF Buffer
  • 5X Phusion GC Buffer
  • DMSO
  • 50 mM MgCl2 solution

Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Phusion Green High-Fidelity DNA Polymerase

  • Phusion DNA Polymerase 2 U/μL
  • 5X Phusion Green HF Buffer
  • 5X Phusion Green GC Buffer
  • DMSO
  • 50 mM MgCl2 solution

Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Product Code. 13575160

Quantity Price
1 £ 99.75 / 100 units
In stock, ready to ship: 8
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Description and Specification

Specification

Content And Storage • Phusion DNA Polymerase, 2 U/μL
• 5X Phusion Green HF and GF Buffers
• DMSO
• 50 mM MgCl2

Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Quantity 100 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR,High-fidelity PCR
For Use With (Application) Standard PCR,High-fidelity PCR
Concentration 2 U/μL
Fidelity (vs. Taq) 52X
Hot Start No
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less
Color Green

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.