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Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up, kit for gel extraction or PCR clean up

Clean up PCR reactions and gel extractions with a two-in-one approach. Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit is optimized for recovery and elution volume when purifying DNA fragments via both applications.

Overview
73.03 GBP valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Code: 86

Additional Details:
Additional Details: Weight: 0.19530kg



Product Code. 12303368

Quantity Price
1 £ 260.0 / 50 reactions
In stock, ready to ship: 50
 In stock, ready to ship: 785
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  • Description and Specification
  • Alternative Products
  • Documentation
  • Accessories and Spare Parts
  • Safety and Handling
Description and Specification

Specification

Color pH Indicator:
Yellow = correct conditions
Green = pH slightly high (adjustment recommended)
Blue = pH too high (adjustment necessary)
Format Mini Spin Column
Isolation Technology Silica Membrane Technology
Efficiency Typical recovery: 70–95%
Elution Volume Elution volume: 15–30μL
For Use With (Equipment) TAE or TBE agarose gels, PCR
Includes Binding Buffer NTI (40mL), Wash Buffer NT3 Concentrate (25mL), Elution Buffer NE (13mL), NucleoSpin Gel and PCR Clean-up Columns (50), 2mL Collection Tubes (50)
No. of Reactions 50 Preps
Quantity 1 Kit
Purity A260/A280: 1.8–1.9
Sample Volume <200 μL PCR reaction mixture, <200 mg agarose gel
Size 50 bp to ∼20 kbp
Test Time 10 min/6 preps
Yield Up to ∼15μg DNA

NucleoSpin™ Gel and PCR Clean-up is designed for fast purification of PCR products, such as DNA from enzymatic reactions, as well as the extraction of DNA fragments from TAE or TBE agarose gels.

  • Two applications in one kit—One buffer to process both sample types, with only one loading step
  • High recovery for fragments down to 50 bp—Average recovery from 60–90%
  • Minimized elution volume of 15μL—Highly concentrated DNA
  • Binding buffer with pH indicator—Ensures optimal binding conditions
  • Separate buffers for single stranded DNA and RNA or for SDS-containing samples available separately
  • Suitable for all kinds of agarose gels and a variety of buffer systems (e.g. TAE, TBE)
  • Complete removal of contaminations: nucleotides, primers, enzymes, mineral oil, PCR additives, detergents, dyes
  • Applications: purification of PCR products, extraction of DNA from agarose or polyacrylamide gels, and downstream applications (cloning, sequencing, PCR, restriction analysis)

Kit contents: NucleoSpin™ Gel and PCR Clean-up columns, collection tubes (2mL), binding buffer with pH indicator, wash buffer and elution buffer

Alternative Products
Documentation

Technical documents

Accessories and Spare Parts
Safety and Handling

[Warning]

[{code=ACUTE TOX. 4, sequence=1, description=Acute toxicity Category 4}, {code=AQUA CHRONIC 3, sequence=2, description=LONG-TERM AQUATIC HAZARD Chronic 3}]

[{code=H302, sequence=1, description=Harmful if swallowed.}, {code=H412, sequence=2, description=Harmful to aquatic life with long lasting effects.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : guanidinium thiocyanate}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H302- Harmful if swallowed.
  • H412- Harmful to aquatic life with long lasting effects.

Supplemental Information
  • MIXTURE LIST- Contains : guanidinium thiocyanate