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Thermo Scientific™ pH Gradient Buffer Concentrates
Robust reproducible gradients that are applicable to a wide range of MAbs and ready to use with existing LC columns and systems, without the need to formulate mobile phases.
Marque: Thermo Scientific™ 083381
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Description
Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.
pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.
- Robust, reproducible gradients
- Predict biomolecule pH
- Simple method optimization
- Easily automated
- Applicable to the majority of MAbs
Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.
Spécification
| CX-1 pH Gradient Starter Kit; 125 mL Buffer A + 125 mL Buffer B MAbPac SCX-10 Column (10 μm, 4 x 250 mm) |
|
| Thermo Scientific | |
| 5.6 to 10.2 |
| LC Columns and Systems | |
| CX-1 pH Gradient Starter Kit | |
| 125 mL + 125 mL |
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