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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Overview
1011.40 GBP valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Additional Details:
Additional Details: Weight: 1.00000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 16254551

Quantity Price
1 £ 1556.0 / 2000 units
In stock, ready to ship: 3
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Description and Specification

Specification

Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Quantity 2000 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR,High-fidelity PCR
For Use With (Application) Standard PCR,High-fidelity PCR
Concentration 2 U/μL
Fidelity (vs. Taq) 52X
Hot Start No
No. of Reactions 2000 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less
Color Colorless

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.