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R&D Systems™ Recombinant Human VEGF 165 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Marca: R&D Systems™ 293-VE-010
377.60 EUR valido fino al 2025-07-30
Usa il codice promo "24078" per avere il tuo prezzo promozionale.
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Descrizione
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286). Specific activity is for reference purposes only and is not routinely tested.
Specifica
| Lyophilized from a 0.2μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. | |
| observed_molecular_weight: 20-22 kDa, under reducing conditions. 39-42 kDa, under non-reducing conditions. | |
| 10 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. | |
| Unconjugated | |
| Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute (at 100-500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.) |
| 7422 | |
| VEGF 165 | |
| Spodoptera frugiperda,Sf 21 (baculovirus)-derived human VEGF protein Ala27-Arg191 | |
| Disulfide-linked homodimer | |
| Recombinant | |
| 97%, by SDS-PAGE with silver staining. |
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