Thermo Scientific™ RapidOut DNA Removal Kit

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations.

Overview
136.85 GBP valid until 2025-06-30
Use promo code "24108" to get your promotional price.



Code: 88

Additional Details:
Additional Details: Weight: 0.04000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes:
  • DNase I, RNase-free (1U/μL) - 250Lμ
  • 10X Reaction Buffer with MgCl2 - 1mL
  • DNase Removal Reagent (DRR) - 500μL
  • Water, nuclease-free - 2 x 1.25mL

Product Code. 13565150

Quantity Price
1 £ 161.0 / 50 tests
In stock, ready to ship: 1
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Description and Specification

Specification

Enzyme DNase
Quantity 50 preps
Product Type Removal Kit

Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations. Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction. First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR. DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR).

DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation. The purified RNA is collected as a supernatant. The RNA after RapidOut procedure is free from DNA contamination and free of DNase I. It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting.

Highlights
Efficient—complete ds and ssDNA digestion and proprietary technology for DNase I removal
Rapid—single step sufficient for complete DNase I removal
Safe—no need for toxic organic extractions or RNA-damaging heating steps

Main Applications
• RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes.
• Customers performing ds-cDNA synthesis from total RNA preps.

Other applications
• Elimination of DNA from RNA for microinjections and transfection.
• Elimination of DNA from RNA prior microarray analysis.
• Elimination of DNA from RNA prior Northern blot analysis.

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