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Gibco™ TrypLE™ Select Enzyme (10X), no phenol red

TrypLE™ Select Enzyme (10X), no phenol red

Overview
140.60 GBP valid until 2025-12-31
Use promo code "24335" to get your promotional price.

Code: 50

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Product Code. 10272625

Quantity Price
1 £ 153.0 / Each
In stock, ready to ship: 134
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Chelators EDTA
Content And Storage Storage conditions: 15–30°C.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 10 X
Culture Type Adherent Cell Culture
Form Liquid
Product Type Cell Culture Dissociation Reagent
Without Additives No Phenol Red
Green Features Energy efficient, Sustainable packaging
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Osmolality 270 - 320 mOsm/kg
pH 6.9 to 7.4
Product Line Gibco™
Purity or Quality Grade Cell Culture Grade
Quantity 100 mL
Remarks Room Temperature Stable
Shipping Condition Room Temperature
Source Animal origin-free
Tested For In vitro Bioassay

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco™ TrypLE™ Select is:

• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:

With
• EDTA

Without
• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco™ TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.

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