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Invitrogen™ PureLink™ Quick Plasmid Miniprep Kit

The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.

Overview
257.02 GBP valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Code: 50

Additional Details:
Additional Details: CAS Number: 9048-46-8 Weight: 1.00000kg Transport: UN number : 3266 Chem class : 8 Pack group : III



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10694823

Quantity Price
1 £ 362.0 / Each
In stock, ready to ship: 16
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
  • Alternative Products
  • Documentation
  • Safety and Handling
Description and Specification

Specification

Concentration 20 mg/mL
Column Type Spin Column
Content And Storage • Resuspension buffer
• RNase A (20 mg/mL)
• Lysis buffer
• Precipitation buffer
• Wash buffers
• TE buffer
• Spin columns in wash tubes
• Elution tubes
Format Spin Column
Isolation Technology Silica Spin Column
Scale Mini
Sample Type Bacterial Culture
Final Product Type Plasmid DNA
For Use With (Application) Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation
High-throughput Compatibility Not High-throughput Compatible (Manual)
No. of Reactions 250 Preps
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
Product Line PureLink™
Product Type Quick Plasmid MiniPrep Kit
Quantity 250 Preps
Shipping Condition Room Temperature
Target Plasmid DNA
Test Time 30 to 45 min.

Features of the PureLink™ Quick Plasmid Miniprep Kit:

  • Fast—ready-to-use plasmid DNA typically in 30-45 minutes
  • Flexible—formats work with vacuum and centrifugation platforms

Reliable and cost-effective isolation of plasmid DNA

The PureLink™ Quick Plasmid Miniprep Kit allows isolation of high-quality plasmid DNA from 1–5 mL cultures in 30-45 minutes, using the PureLink™ spin columns. These columns use a unique silica membrane to achieve high yields of up to 40 μg of sequencing-grade plasmid DNA. Cells are lysed using an alkaline/SDS procedure and the lysate is applied to a silica membrane column that selectively binds plasmid DNA. Contaminants are removed with Wash Buffer, and the plasmid DNA is eluted in TE Buffer. The eluted DNA is suitable for all routine downstream applications.

Compatible with flexible formats

The PureLink™ Quick Plasmid Miniprep Kit can be used with a centrifuge or a vacuum manifold for purifying plasmid DNA. Typically, DNA isolated using the PureLink™ Quick Plasmid Miniprep Kit has an A260/A280 of >1.80 when samples are diluted in Tris-HCl (pH 7.5), indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.

Isolated DNA can be used for a variety of downstream applications

Plasmid DNA purified using PureLink™ Quick Plasmid Miniprep Kit is suitable for all routine downstream applications, including bacterial and mammalian cell transformations, DNA sequencing, restriction enzyme digestion, cloning, and PCR.

Alternative Products
Documentation

Technical documents

Safety and Handling

[PureLink Quick Plasmid Miniprep Kit]

[Warning]

[{code=EYE DAM. 2, sequence=1, description=Serious eye damage/eye irritation Category 2}, {code=SKIN IRRIT. 2, sequence=2, description=Skin corrosion/irritation Category 2}]

[{code=H315, sequence=1, description=Causes skin irritation.}, {code=H319, sequence=2, description=Causes serious eye irritation.}]

[{code=P264, sequence=51, description=Wash hands thoroughly after handling.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P302+P352, sequence=53, description=IF ON SKIN: Wash with plenty of water/soap.}, {code=P305+P351+P338, sequence=54, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}, {code=P332+P313, sequence=55, description=If skin irritation occurs: Get medical advice/attention.}, {code=P337+P313, sequence=56, description=If eye irritation persists: Get medical advice/attention.}, {code=P362+P364, sequence=57, description=Take off contaminated clothing and wash it before reuse.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Acetic acid}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Product Identifier
  • PureLink Quick Plasmid Miniprep Kit

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315- Causes skin irritation.
  • H319- Causes serious eye irritation.

Precautionary Statement
  • P264- Wash hands thoroughly after handling.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P302+P352- IF ON SKIN: Wash with plenty of water/soap.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P332+P313- If skin irritation occurs: Get medical advice/attention.
  • P337+P313- If eye irritation persists: Get medical advice/attention.
  • P362+P364- Take off contaminated clothing and wash it before reuse.

Supplemental Information
  • MIXTURE LIST- Contains : Acetic acid