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Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

Overview
47.22 GBP valid until 2025-04-18
Use promo code "24107" to get your promotional price.

Code: 88

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10849700

Quantity Price
1 £ 66.5 / 1000 units
In stock, ready to ship: 4
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Description and Specification

Specification

Concentration 1 U/μL
Enzyme DNase
Compatible Buffer Reaction Buffer
Quantity 1,000 units
Product Type DNase I RNase Free

Highlights

  • Recombinant enzyme
  • Purified from non-animal host with a lower level of intrinsic RNases

Applications

  • Preparation of DNA-free RNA
  • Removal of template DNA followingin vitrotranscription
  • Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
  • DNA labeling by nick-translation in conjunction with DNA Polymerase I
  • Studies of DNA-protein interactions by DNase I, RNase-free footprinting
  • Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.