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Gibco™ RPMI 1640 Medium, powder, HEPES

RPMI 1640 Medium, powder, HEPES

Overview
Code: 50

Additional Details:
Additional Details: Weight: 0.01100kg



Product Code. 11500456

Quantity Price
1 £ 77.75 / 10 L
In stock, ready to ship: 1
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
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Description and Specification

Specification

Form Powder
Product Type RPMI 1640 Medium
With Additives Glutamine,HEPES,Phenol Red
With Additives Glutamine,HEPES,Phenol Red
With Additives Glutamine,HEPES,Phenol Red
Without Additives No Sodium Bicarbonate,No Sodium Pyruvate
Without Additives No Sodium Bicarbonate,No Sodium Pyruvate
For Use With (Application) Mammalian cell culture
Quantity 10 L
Certifications/Compliance ISO 13485 standards, FDA
Regulatory Status For in-vitro diagnostic use
Content And Storage 2 to 8°C
Description RPMI 1640 Medium, 10L, Powdered Form, With L-glutamine, Phenol Red, HEPES, 36 Months Shelf Life, 2 to 8°C Storage Conditions, Used for Mammalian Cell Culture

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12, and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco™ RPMI 1640 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, Life Technologies offers a comparable Gibco™ RPMI 1640 product made in our Grand Island facility (23400-021). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ L-15 cell culture medium require sodium bicarbonate supplementation pH adjustment, and filtration at the time of preparation.

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