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Thermo Scientific™ HisPur™ Ni-NTA Superflow Agarose

Perform FPLC purification of poly-histidine-tagged proteins with this nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2).

Overview
509.20 GBP valid until 2025-12-31
Use promo code "24353" to get your promotional price.

Code: PN

Additional Details:
Additional Details: CAS Number: 10101-97-0 Weight: 0.05000kg Transport: UN number : 1993 Chem class : 3 Pack group : III



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 13404199

Quantity Price
1 £ 566.0 / 50mL
In stock, ready to ship: 2
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
  • Special Offers
Description and Specification

Specification

Capacity (Metric) 20 mg His-Tagged GFP Binding per mL Resin
Content And Storage Store at 4°C
Form Liquid Suspension
Product Line HisPur™
Quantity 50 mL
Target His-Tagged Protein
Type Agarose
Stationary Phase Divalent Nickel, Metal Chelate
Format Bottle
Column Type Affinity, Agarose Resin

HisPur Ni-NTA Superflow Agarose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification. HisPur Ni-NTA Superflow Agarose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Highlights:

  • High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

  • High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

  • Versatile – can be used to purify proteins under native and denaturing conditions

  • Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

  • Compatible – maintains function in a wide variety of chemicals and pH conditions

  • Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Recommended for:

Medium- to large-scale FPLC purification of polyhistidine tagged proteins

Alternative Products
Safety and Handling

[HisPur Ni-NTA Superflow Agarose, 50 mL.]

[Danger]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=SKIN SENS. 1, sequence=2, description=Skin sensitiser Category 1}, {code=RESP. SENS. 1, sequence=3, description=Respiratory sensitiser Category 1}, {code=CARC. 1, sequence=4, description=Carcinogenicity Category 1}, {code=REPR. 1B, sequence=5, description=Reproductive toxicity Category 1B}, {code=STOT RE 2, sequence=6, description=Specific target organ toxicity after repeated exposure Category 2}, {code=AQUA CHRONIC 3, sequence=7, description=LONG-TERM AQUATIC HAZARD Chronic 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H317, sequence=2, description=May cause an allergic skin reaction.}, {code=H334, sequence=3, description=May cause allergy or asthma symptoms or breathing difficulties if inhaled.}, {code=H350, sequence=4, description=May cause cancer.}, {code=H360D, sequence=5, description=May damage the unborn child.}, {code=H373, sequence=6, description=May cause damage to organs.}, {code=H412, sequence=7, description=Harmful to aquatic life with long lasting effects.}]

[{code=P201, sequence=51, description=Obtain special instructions before use.}, {code=P260, sequence=52, description=Do not breathe dust/fume/gas/mist/vapours/spray.}, {code=P302+P352, sequence=53, description=IF ON SKIN: Wash with plenty of water/.}, {code=P304+P340, sequence=54, description=IF INHALED: Remove person to fresh air and keep comfortable for breathing.}, {code=P303+P361+P353, sequence=55, description=IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.}, {code=P370+P378, sequence=56, description=In case of fire: Use to extinguish.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Ethyl alcohol, Nickel(II) sulfate hexahydrate(1:1:6)}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS08, sequence=2, description=HEALTH HAZARD}]


Product Identifier
  • HisPur Ni-NTA Superflow Agarose, 50 mL.

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Carcinogenicity Category 1
  • Reproductive toxicity Category 1B
  • Specific target organ toxicity after repeated exposure Category 2
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H317- May cause an allergic skin reaction.
  • H334- May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350- May cause cancer.
  • H360D- May damage the unborn child.
  • H373- May cause damage to organs.
  • H412- Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P201- Obtain special instructions before use.
  • P260- Do not breathe dust/fume/gas/mist/vapours/spray.
  • P302+P352- IF ON SKIN: Wash with plenty of water/.
  • P304+P340- IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P303+P361+P353- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P370+P378- In case of fire: Use to extinguish.

Supplemental Information
  • MIXTURE LIST- Contains : Ethyl alcohol, Nickel(II) sulfate hexahydrate(1:1:6)
Special Offers