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Invitrogen™ Platinum™ SuperFi II DNA Polymerase

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Overview
657.36 GBP valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Additional Details:
Additional Details: Weight: 0.32000kg



Product Code. 16552642

Quantity Price
1 £ 986.0 / 500 reactions
In stock, ready to ship: 3
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  • Description and Specification
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Description and Specification

Specification

Content And Storage • Platinum SuperFi II DNA Polymerase (500 μL)
• 5X SuperFi II Buffer (6 x 1.25 mL)

Store at –20°C.
GC-Rich PCR Performance High
Polymerase Platinum SuperFi II DNA Polymerase
Reaction Speed Fast
Quantity 500 reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR,High-fidelity PCR
For Use With (Application) Hot-start PCR,High-fidelity PCR
Fidelity (vs. Taq) >300X
Hot Start Built-In Hot Start
No. of Reactions 500 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less

Features of Platinum SuperFi II DNA Polymerase include:

  • Exceptional >300X Taq fidelity
  • Universal primer annealing at 60°C
  • Superior specificity, sensitivity, and yields
  • Robust amplification of difficult-to-amplify targets

Platinum SuperFi II DNA Polymerase is an engineered enzyme with high processivity and increased resistance to PCR inhibitors. It also enables fast-cycling protocols and amplification of long targets (up to 20 kb). Platinum hot-start technology is based on proprietary antibodies that inhibit enzyme activity until the initial PCR denaturation step, preventing non-specific amplification and primer degradation. This technology also enables reaction setup at room temperature and provides increased sensitivity and yield.

Isostabilizing molecules in the buffer increase primer-template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize the annealing temperature for each primer pair. With Platinum SuperFi II DNA Polymerase, different PCR assays can be cycled together using the same protocol with a universal primer annealing temperature and an extension step selected for the longest fragment to be amplified.

Applications

  • High-fidelity PCR
  • Cloning and sub-cloning
  • Site-directed mutagenesis
  • Amplification of GC-rich templates
  • Template generation for sequencing
  • High-throughput PCR
  • Amplification of samples with suboptimal purity
  • Long PCR

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