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Applied Biosystems™ SYBR™ Green Universal Master Mix

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

£118.0 - £3555.0

See promotional offers below

Specification

Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
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Disclaimers: For Research Use Only. Not for use in diagnostic procedures.
Includes: 2X mixture of SYBR Green I Dye, AmpliTaq Gold DNA Polymerase, dNTPs with dUTP, Passive Reference 1 (ROX), and optimized buffer components.


Specification

Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Label or Dye SYBR Green
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR
Product Type Real Time PCR SYBR Master Mix
For Use With (Application) Gene Expression
Concentration

  • SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required
  • AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance
  • dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
  • Proprietary buffer enhancements ensure performance and reliability
  • Premixed components stored at 2° to 8°C significantly reduce assay setup time

Maximum Flexibility and Convenience:

  • Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required
  • SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR
  • The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity
  • Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation
  • SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
  • Store at -20°C

Order Info Shipping Condition: Wet Ice

  Product Code Brand Quantity Price ??product.searchResults.label.quantityAvail??
Quantity
10607705
Applied Biosystems™
4344463
 1 x 1 mL £118.0
Each
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10187094
Promotion Details
Applied Biosystems™
4309155
 1 x 5 mL £418.0
Each
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10418454
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Applied Biosystems™
4364344
 2 x 5 mL £806.0
1 set
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10115155
Applied Biosystems™
4364346
 5 x 5 mL £1894.0
1 set
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10666175
Applied Biosystems™
4312704
 10 x 5 mL £3555.0
1 set
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10565385
Applied Biosystems™
4334973
 1 x 50 mL £3275.0
Each
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** 10177204
Applied Biosystems™
4364346
 5 x 5 mL n/a  
** No longer available. See item for replacement.