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Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Overview
1025.65 GBP valid until 2025-12-31
Use promo code "25339" to get your promotional price.

Code: 88

Additional Details:
Additional Details: Weight: 4.32270kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10171080

Quantity Price
1 £ 1134.0 / Each
In stock, ready to ship: 2
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Content And Storage • 5 x 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 200 mL Lysis Buffer; room temperature
• 200 mL Wash Buffer 1 (concentrated); room temperature
• 100 mL Wash Buffer 2 (concentrated); room temperature
• 125 mL Nuclease-free Water; room temperature
• 250 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 250 Collection Tubes (2 mL); room temperature
• 250 Collection Tubes (1.5 mL); room temperature
Isolation Technology Silica Spin Column
Purification Time 15 min. (After sample homogenization)
Elution Volume 50–100 μL
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity 250 Preps
Scale Mini
Shipping Condition Room Temperature
Starting Material Amount Bacteria: ≤109 cells, Blood: 0.5 mL, Cells: ≤107, Tissue and Insects: 30 mg, Yeast: ≤4 x 108 cells, Enzymatic reactions: ≤100 μL
Yield Up to 160 μg

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

  • Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
  • Efficient—high yields of total RNA
  • Fast—15 minutes after lysis step
  • Pure—an A260/280 ratio > 1.9
  • Convenient—spin columns are capped and assembled with collection tubes

Applications

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:

  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assay

Includes

Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

Alternative Products
Safety and Handling

[GeneJET RNA Purification Kit]

[Danger]

[{code=ACUTE TOX. 4, sequence=1, description=Acute toxicity Category 4}, {code=AQUA CHRONIC 3, sequence=2, description=LONG-TERM AQUATIC HAZARD Chronic 3}, {code=EYE DAM. 2, sequence=3, description=Serious eye damage/eye irritation Category 2}, {code=RESP. SENS. 1, sequence=4, description=Respiratory sensitiser Category 1}, {code=SKIN CORR. 1C, sequence=5, description=Skin corrosion/irritation Category 1 C}, {code=SKIN IRRIT. 2, sequence=6, description=Skin corrosion/irritation Category 2}]

[{code=H302, sequence=1, description=Harmful if swallowed.}, {code=H314, sequence=2, description=Causes severe skin burns and eye damage.}, {code=H315, sequence=3, description=Causes skin irritation.}, {code=H317, sequence=4, description=May cause an allergic skin reaction.}, {code=H319, sequence=5, description=Causes serious eye irritation.}, {code=H412, sequence=6, description=Harmful to aquatic life with long lasting effects.}]

[{code=P261, sequence=51, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P264, sequence=52, description=Wash hands thoroughly after handling.}, {code=P270, sequence=53, description=Do not eat, drink or smoke when using this product.}, {code=P273, sequence=54, description=Avoid release to the environment.}, {code=P280, sequence=55, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P284, sequence=56, description=¢In case of inadequate ventilation! wear respiratory protection.}, {code=P301+P310, sequence=57, description=IF SWALLOWED: Immediately call a POISON CENTER/doctor /}, {code=P302+P352, sequence=58, description=IF ON SKIN: Wash with plenty of water/soap.}, {code=P303+P361+P353, sequence=59, description=IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.}, {code=P304+P340, sequence=60, description=IF INHALED: Remove person to fresh air and keep comfortable for breathing.}, {code=P305+P351+P338, sequence=61, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}, {code=P330, sequence=62, description=Rinse mouth.}, {code=P332+P313, sequence=63, description=If skin irritation occurs: Get medical advice/attention.}, {code=P337+P313, sequence=64, description=If eye irritation persists: Get medical advice/attention.}, {code=P342+P311, sequence=65, description=If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .}, {code=P362+P364, sequence=66, description=Take off contaminated clothing and wash it before reuse.}, {code=P501b, sequence=67, description=Dispose of contents/container in accordance with local/regional/national/international regulations.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Thiocyanic acid, Guanidine hydrochloride, Proteinase}]

[{code=GHS05, sequence=1, description=CORROSION}, {code=GHS07, sequence=2, description=EXCLAMATION MARK}, {code=GHS08, sequence=3, description=HEALTH HAZARD}]


Product Identifier
  • GeneJET RNA Purification Kit

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 4
  • LONG-TERM AQUATIC HAZARD Chronic 3
  • Serious eye damage/eye irritation Category 2
  • Respiratory sensitiser Category 1
  • Skin corrosion/irritation Category 1 C
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H302- Harmful if swallowed.
  • H314- Causes severe skin burns and eye damage.
  • H315- Causes skin irritation.
  • H317- May cause an allergic skin reaction.
  • H319- Causes serious eye irritation.
  • H412- Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P264- Wash hands thoroughly after handling.
  • P270- Do not eat, drink or smoke when using this product.
  • P273- Avoid release to the environment.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P284- ¢In case of inadequate ventilation! wear respiratory protection.
  • P301+P310- IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P302+P352- IF ON SKIN: Wash with plenty of water/soap.
  • P303+P361+P353- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340- IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P330- Rinse mouth.
  • P332+P313- If skin irritation occurs: Get medical advice/attention.
  • P337+P313- If eye irritation persists: Get medical advice/attention.
  • P342+P311- If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
  • P362+P364- Take off contaminated clothing and wash it before reuse.
  • P501b- Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental Information
  • MIXTURE LIST- Contains : Thiocyanic acid, Guanidine hydrochloride, Proteinase