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Thermo Scientific™ GeneJET RNA Purification Kit

Thermo Scientific GeneJET RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria, and yeast.

Overview
Code: 88

Additional Details:
Additional Details: Weight: 0.78471kg Transport: UN number : 1760 Chem class : 8 Pack group : III



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10558360

Quantity Price
1 £ 253.0 / Each
In stock, ready to ship: 4
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  • Description and Specification
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Description and Specification

Specification

Content And Storage • 600 μL Proteinase K; store at room temperature until opened, -20°C after opening
• 40 mL Lysis Buffer; room temperature
• 40 mL Wash Buffer 1 (concentrated); room temperature
• 23 mL Wash Buffer 2 (concentrated); room temperature
• 30 mL Nuclease-free Water; room temperature
• 50 GeneJET RNA Spin Columns with Collection Tubes; For better long-term performance, it is recommended to store the purification columns at 2°C-8°C.
• 50 Collection Tubes (2 mL); room temperature
• 50 Collection Tubes (1.5 mL); room temperature
Isolation Technology Silica Spin Column
Purification Time 15 min. (After sample homogenization)
Elution Volume 50–100 μL
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Sample Type Bacteria,Blood,Cells,Tissue,Yeast,Insects,Enzymatic Reactions
Final Product Type Total RNA
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Quantity 50 Preps
Scale Mini
Shipping Condition Room Temperature
Starting Material Amount Bacteria: ≤109 cells, Blood: 0.5 mL, Cells: ≤107, Tissue and Insects: 30 mg, Yeast: ≤4 x 108 cells, Enzymatic reactions: ≤100 μL
Yield Up to 160 μg

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation, or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications, including RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analyses.

Highlights

  • Universal—can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, and bacteria
  • Efficient—high yields of total RNA
  • Fast—15 minutes after lysis step
  • Pure—an A260/280 ratio > 1.9
  • Convenient—spin columns are capped and assembled with collection tubes

Applications

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:

  • RT-PCR
  • RT-qPCR
  • Northern blotting
  • Nuclease protection assay

Includes

Proteinase K Lysis Buffer, Wash Buffer 1 (concentrated), Wash Buffer 2 (concentrated), Nuclease-free Water, GeneJET RNA Purification Columns (pre-assembled with Collection Tubes), Collection Tubes (2 mL), Collection Tubes (1.5 mL), Detailed Protocol

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