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Cytiva HisTrap™ excel Prepacked Columns

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. Cytiva Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Overview
Code: GE

Additional Details:
Additional Details: Weight: 0.13500kg



Product Code. 12683466

Quantity Price
1 £ 748.25 / Pack of 5
In stock, ready to ship: 9
 In stock, ready to ship: 65
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Description and Specification

Specification

Bed Volume 5 mL
Diameter (English) Inner 0.6 in.
Diameter (Metric) Inner 16 mm
Description HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
Flow Rate 5 to 20 mL/min.
Material Polypropylene
pH Range 2 to 12
Support Type 6% Highly Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Quantity 5 x 5 mL
Type Pre-packed Column

The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

  • Ready-to use prepacked with Ni Sepharose excel
  • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
  • Made of biocompatible polypropylene and cannot be opened or refilled
  • Delivered with stopper on inlet and snap-off end on outlet
  • Operated either with peristaltic pump or with chromatography systems such as KTA systems
  • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
Media
  • Average particle size: 90μm
  • Matrix: Highly cross-linked agarose, 6%
  • pH Stability working range: 2 to 12
  • pH Stability cleaning range: 2 to 14
  • Flow velocity: 150 cm/h - 600 cm/h4
Column:
  • Column I.D.: 7 mm

HisTrap excel 1mL column

  • Bed Dimensions: 7 × 25mm
  • Flow rate: 1 to 4mL/min.
HisTrap excel 5mL column
  • Bed Dimensions: 16 × 25mm
  • Flow rate: 5 to 20mL/min.

Safety and Handling

[Warning]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=EUH208, sequence=91, description=Contains nickel. May produce an allergic reaction.}]

[{code=P280, sequence=51, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P210, sequence=52, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P241, sequence=53, description=Use explosion-proof electrical/ventilating/lighting/ equipment.}, {code=P303+P361+P353, sequence=54, description=IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.}, {code=P235, sequence=55, description=Keep cool.}, {code=P501b, sequence=56, description=Dispose of contents/container in accordance with local/regional/national/international regulations.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Ethanol, Nickel}]

[{code=GHS02, sequence=1, description=FLAME}]


Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3

Hazard Statement
  • H226- Flammable liquid and vapour.
  • EUH208- Contains nickel. May produce an allergic reaction.

Precautionary Statement
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241- Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353- IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235- Keep cool.
  • P501b- Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental Information
  • MIXTURE LIST- Contains : Ethanol, Nickel