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Gibco™ Advanced DMEM/F-12

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation.

Overview
215.20 GBP valid until 2025-12-31
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Code: 50

Additional Details:
Additional Details: Weight: 6.34000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 11550446

Quantity Price
1 £ 287.0 / Pack of 10
In stock, ready to ship: 78
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For Research Use Only. All usage must comply with product instructions.
 

  • Description and Specification
  • Alternative Products
  • Safety and Handling
  • Special Offers
Description and Specification

Specification

Cell Line MRC-5, SP2, Vero, WI-38 and Jurkat
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type DMEM (Dulbecco's Modified Eagle Medium)/F-12
Serum Level Reduced Serum
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
With Additives High Glucose,Phenol Red,Sodium Pyruvate,Non Essential Amino Acids (NEAA)
Without Additives No Glutamine,No HEPES
Without Additives No Glutamine,No HEPES
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Product Line Gibco™
Quantity 10 x 500 mL
Shipping Condition Room Temperature
Content And Storage Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Advanced DMEM/F-12 (Dulbecco's Modified Eagle Medium/Ham's F-12) is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F-12, serum supplementation can be reduced by 50–90% with no change in growth rate or morphology. Cells successfully cultured in Advanced DMEM/F-12, with no adaptation, include MRC-5, SP2, Vero, WI-38 and Jurkat.

This Advanced DMEM/F-12 is manufactured as follows:

With: Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Using Advanced DMEM/F-12

Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ II lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 requires supplementation with 1–5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

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Safety and Handling

[Methyl benzoate]

[Warning]

[{code=ACUTE TOX. 4, sequence=1, description=Acute toxicity Category 4}]

[{code=H302, sequence=1, description=Harmful if swallowed.}]

[{code=P301+P312, sequence=51, description=IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : Methyl benzoate}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Product Identifier
  • Methyl benzoate

Signal Word
  • Warning

Hazard Category
  • Acute toxicity Category 4

Hazard Statement
  • H302- Harmful if swallowed.

Precautionary Statement
  • P301+P312- IF SWALLOWED: Call a POISON CENTER or doctor/physician/ if you feel unwell.

Supplemental Information
  • MIXTURE LIST- Contains : Methyl benzoate
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