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Invitrogen™ iBlot™ 2 Gel Transfer Device

Performs Western blotting transfer simply, efficiently, and reliably, within seven minutes and without the need for liquid buffers

Overview

Code: NEW

Additional Details:
Additional Details: Weight: 0.09000kg



Requires: iBlot 2 device and transfer stacks
Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15217995

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  • Description and Specification
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Description and Specification

Specification

Content And Storage Store at room temperature.
For Use With (Application) Western Blot
For Use With (Equipment) iBlot™
Gel Compatibility Bolt Bis-Tris Plus Gel, E-PAGE Gel, Novex Midi Gel, NuPAGE Gel, Novex Mini Gel
Membrane Compatibility Nitrocellulose, PVDF
Mode of Transfer Dry
Product Line iBlot
Running Time ∼7 min.
Throughput 1 Midi or 2 Mini Gels
Type Gel Transfer Device
Quantity 1 Device
Running Dimension Horizontal

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF).

  • Complete protein transfer in 7 minutes or less
  • High detection sensitivity and even transfer
  • Increased blotting reliability and reproducibility
  • Flexible gel size formats and membrane types
  • A simple, user-friendly system
  • Options to create new custom programs
  • Built-in tutorial and application notes
  • High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

  • For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
  • Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
  • Allows users to create custom programs
iBlot 2 Transfers Save Time
  • With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
  • The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques
How It Works
  • Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
  • The high density of ions in the gel matrix enables rapid protein transfer
  • During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
  • Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
  • Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

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