Invitrogen™ Qubit™ 3 Fluorometer

The fluorescent dyes used in the Qubit assays emit signals only when bound to specific target molecules, even at low concentrations, thus minimizing the effects of contaminants, including degraded DNA or RNA. The integrated design of the instrument and assays results in quantitation that is far more sensitive than UV absorbance, making this system ideal for quantitation of precious samples (samples that are rare, difficult to purify, or expensive to either obtain or prepare) or samples for delicate applications (samples for downstream assays that are extremely sensitive to sample conditions).

Key Features

• Powerful dual-core processor quickly and accurately quantifies DNA, RNA, and protein in less than 5 seconds per sample
• Uses samples as little as 1μL up to 20μL
• Stores up to 1000 sample results
• Large, 5.7-inch, state-of-the-art color touch screen for easy workflow navigation
• Ability to personalize Qubit fluorometer with the frequently run assays, add new assays, or even create custom assays with the MyQubit software and web tool
• Small footprint saves bench space
• Language options include English, French, Spanish, German, simplified Chinese and Japanese
• Android-based operating system

More Accurate than UV Absorbance

• More sensitive than UV absorbance, which measures anything absorbing at 260nm, including DNA, RNA, protein, free nucleotides, or excess salts
• Additionally, UV spectrophotometry is often not sensitive enough to accurately measure low concentrations of DNA and RNA
• The Qubit Fluorometer provides more accuarate measurements becuase the dyes in the Qubit assay kits fluoresce only when bound to the selected molecule—DNA, RNA, or protein—in a sample

• Calculations and settings are automatically performed by the instrument
• Qubit assays for use with the fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes
• Advanced optics and data analysis algorithms
• USB thumb drive and cable for data transfer to Excel™ sorftware and for software downloads

Applications:

Measurement of DNA, RNA, and protein using Qubit quantitation assays; Direct measurement of the fluorescence of samples; Evaluation of Ion Sphere™ Particle quality using the Ion Sphere Quality Control Kit prior to performing a sequencing run on the Ion PGM™ Sequencer