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Invitrogen™ Lipofectamine™ 3000 Transfection Reagent

Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

Overview
829.60 GBP valid until 2025-12-31
Use promo code "24071" to get your promotional price.

Code: NEW

Additional Details:
Additional Details: Weight: 0.10000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 15212475

Quantity Price
1 £ 1002.0 / 1.50mL
In stock, ready to ship: 26
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  • Description and Specification
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Description and Specification

Specification

Classification Animal Origin-Free
Content And Storage Contains one vial of Lipofectamine™ 3000 Reagent (1.5 mL) and one vial of P3000TM Enhancer Reagent (1.5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. Store at 2–8°C. Do not freeze.
Cell Type Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells
Cell Type Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells
Cell Type Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells
Cell Type Established Cell Lines,Stem Cells,Primary Cells,Hard-to-Transfect Cells
Format 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample Type Plasmid DNA,Synthetic siRNA,RNAi Plasmids (shRNA, miR)
Sample Type Plasmid DNA,Synthetic siRNA,RNAi Plasmids (shRNA, miR)
Sample Type Plasmid DNA,Synthetic siRNA,RNAi Plasmids (shRNA, miR)
Transfection Technique Lipid-based Transfection
For Use With (Application) Transfection
Product Line Lipofectamine
Product Type Transfection Reagent
Quantity 1.5 mL
Serum Compatible Yes

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
  • Superior performance—our highest efficiency reagent for difficult-to-transfect cells
  • Improved cell viability—gentle on your cells, with low toxicity
  • Versatile—one reagent for DNA, RNA, and co-transfection

Efficiently Transfect Difficult-to-Transfect Cells
Lipofectamine 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

Fibroblast (3T3, COS-7)
Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7)
Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T)
Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460)
Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480)
Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)

Transfect Common Cells Efficiently and Cost-Effectively
For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The 1.5mL size provides up to 1500 transfection reactions (in 24-well plates).

Achieve Superior Transfection Efficiency While Maintaining Low Toxicity
Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. A low toxicity lipid dose (0.75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells.

Enable High Transfection Efficiency in Novel Genome Editing Applications
Lipofectamine 3000 reagent is suitable for novel genome editing applications. It increases the likelihood of successful cleavage and recombination with TALENs or CRISPRs through highly efficient transfection, ultimately maximizing the efficiency of genetic modifications and simplifying the downstream processes.

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