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Cytiva Ni Sepharose™ 6 Fast Flow

Combines the advantages of Ni2 for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform. Cytiva Lifescience™ Ni Sepharose™ 6 Fast Flow is part of a range of IMAC chromatography media for capture and intermediate purification.

Overview
Code: GE

Additional Details:
Additional Details: CAS Number: 64-17-5 Weight: 0.79800kg



Product Code. 10353060

Quantity Price
1 £ 4947.0 / 500mL
Estimated Shipment
10-06-2025
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  • Description and Specification
  • Alternative Products
  • Safety and Handling
Description and Specification

Specification

Buffer 0.1M HCl, 0.1M NaOH, 8M Urea
Product Type Ni Sepharose 6 FF chromatography media
Quantity 500 mL
Height (English) 9.84 in.
Height (Metric) 250mm
Flow Rate 600 cm/hr.
Max. Pressure 1 bar
pH Range 3 to 12
Particle Size 90μm
Support Type 6% Cross-linked Agarose
Content And Storage 4° to 30°C
dimMatrix Ni-Sepharose

Ni Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

  • Designed for scaling up purification of histidine-tagged proteins.
  • High protein binding capacity and minimal leakage of Ni2+ ions.
  • As a BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in convenient prepacked HisPrep and HisTrap column formats.
  • Composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2+ ions for purification of histidine tagged proteins.
  • The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2+ leakage while providing high capacity.
  • Available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
  • As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Alternative Products
Safety and Handling

[Warning]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=AQUATIC ACUTE 1, sequence=2, description=ACUTE AQUATIC Acute 1}, {code=AQUA CHRONIC 3, sequence=3, description=LONG-TERM AQUATIC HAZARD Chronic 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H400, sequence=2, description=Very toxic to aquatic life.}, {code=H412, sequence=3, description=Harmful to aquatic life with long lasting effects.}]

[{code=P210, sequence=51, description=Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.}, {code=P241, sequence=52, description=Use explosion-proof electrical/ventilating/lighting/ equipment.}, {code=P273, sequence=53, description=Avoid release to the environment.}, {code=P501b, sequence=54, description=Dispose of contents/container in accordance with local/regional/national/international regulations.}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS09, sequence=2, description=ENVIRONMENT}]


Signal Word
  • Warning

Hazard Category
  • Flammable liquid Category 3
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H400- Very toxic to aquatic life.
  • H412- Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P210- Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241- Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P273- Avoid release to the environment.
  • P501b- Dispose of contents/container in accordance with local/regional/national/international regulations.