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Macherey-Nagel™ NucleoSpin™ RNA, Mini kit for RNA purification

Mini spin kit for isolation of RNA of highest integrity.

Additional Details:
Additional Details: Weight: 0.01000kg



Product Code. 15220023

Quantity Price
1 £ 1180.0 / Each
Estimated Shipment
04-06-2025
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Description and Specification

Specification

Bacterial Content 70 μg
Detection Method Silica-membrane
Format QIAcube
Isolation Technology Silica Membrane
DNase Yes
Final Product Type NucleoSpin™ RNA Kit
For Use With (Application) RNA isolation from cultured cells, tissue
No. of Reactions 240
No. of Samples 4
Product Type NucleoSpin™ RNA Kit
Quantity 1 Each
Sample Size <5 x 106 cultured cells, <30 mg human/animal tissue, <109 bacterial cells, <108 yeast cells
Yield 14μg HeLa cells, 70μg Bacterial Cells

Features
  • DNase included for on-column DNA digestion– easy and efficient removal of contaminating DNA
  • NucleoSpin™ Filters (shredders) – efficient sample homogenization and reduction of viscosity
  • Up to 70 μg ready-to-use RNA
  • Parallel purification of genomic DNA by using the NucleoSpin™ RNA/DNA Buffer Set
Applications
  • RNA isolation from cultured cells, tissue (standard protocol)
  • RNA from < 109 bacterial cells (Gram-negative, Gram-positive) or < 108 yeast cells
  • RNA from ≤ 100 μL biological fluids
  • RNA clean-up from reaction mixtures
  • RNA from samples stored in RNAlater™
  • RNA from saliva samples collected with Oragene™ RNA (Genotek)
  • Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology, RNase protection assays

Safety and Handling

[Danger]

[{code=FLAM. LIQ. 3, sequence=1, description=Flammable liquid Category 3}, {code=ACUTE TOX. 4, sequence=2, description=Acute toxicity Category 4}, {code=RESP. SENS. 1, sequence=3, description=Respiratory sensitiser Category 1}, {code=AQUA CHRONIC 3, sequence=4, description=LONG-TERM AQUATIC HAZARD Chronic 3}]

[{code=H226, sequence=1, description=Flammable liquid and vapour.}, {code=H302, sequence=2, description=Harmful if swallowed.}, {code=H334, sequence=3, description=May cause allergy or asthma symptoms or breathing difficulties if inhaled.}, {code=H412, sequence=4, description=Harmful to aquatic life with long lasting effects.}]

[{code=P261, sequence=51, description=Avoid breathing dust/fume/gas/mist/vapours/spray.}, {code=P342+P311, sequence=52, description=If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .}]

[{code=MIXTURE LIST, sequence=1, description=Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) / guanidine hydrochloride, ethanol}]

[{code=GHS02, sequence=1, description=FLAME}, {code=GHS07, sequence=2, description=EXCLAMATION MARK}, {code=GHS08, sequence=3, description=HEALTH HAZARD}]


Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 3
  • Acute toxicity Category 4
  • Respiratory sensitiser Category 1
  • LONG-TERM AQUATIC HAZARD Chronic 3

Hazard Statement
  • H226- Flammable liquid and vapour.
  • H302- Harmful if swallowed.
  • H334- May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H412- Harmful to aquatic life with long lasting effects.

Precautionary Statement
  • P261- Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P342+P311- If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .

Supplemental Information
  • MIXTURE LIST- Contains : guanidinium thiocyanate, ethanol (diluted < 20 %) / guanidine hydrochloride, ethanol