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Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Overview
Code: 87

Additional Details:
Additional Details: Weight: 0.02000kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10024537

Quantity Price
1 £ 103.0 / 100 units
In stock, ready to ship: 6
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  • Description and Specification
  • Alternative Products
  • Documentation
Description and Specification

Specification

Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion HF & GC Buffers
• DMSO
• 50 mM MgCl2 solution

Phusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
GC-Rich PCR Performance High
Polymerase Phusion High-Fidelity DNA Polymerase
Reaction Speed Fast
Product Type High-Fidelity DNA Polymerase
Quantity 100 units
Shipping Condition Dry Ice
For Use With (Application) Standard PCR,High-fidelity PCR
For Use With (Application) Standard PCR,High-fidelity PCR
Concentration 2 U/μL
Fidelity (vs. Taq) 52X
Hot Start No
No. of Reactions 100 Reactions
Overhang Blunt
Reaction Format Standalone
Size (Final Product) 20 kb or less
Color Colorless

Features of Phusion™ High-Fidelity DNA Polymerase include:

  • High fidelity (52X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed
  • High yields of PCR products with minimal enzyme amounts
  • Available in Green buffer format for direct loading of PCR products on gels (F-534S or F-534L)

Applications

  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Amplification of difficult (GC-rich) templates
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR
  • Microarray

Note: Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases). For optimal results, use our convenient Tm calculator.

Alternative Products
Documentation

Technical documents