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Thermo Scientific™ Subcellular Protein Fractionation Kit for Cultured Cells

Enrich for cultured cell-specific cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins in less than 3 hours.

Overview
422.68 GBP valid until 2025-06-21
Use promo code "24112" to get your promotional price.

Code: PN

Additional Details:
Additional Details: Weight: 1.36080kg



Disclaimers: For Research Use Only. Not for use in diagnostic procedures.

Product Code. 10455924

Quantity Price
1 £ 634.0 / 1 set
In stock, ready to ship: 8
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  • Description and Specification
  • Alternative Products
  • Documentation
  • Safety and Handling
  • Special Offers
Description and Specification

Specification

Product Type Subcellular Protein Fractionation Kit
Content And Storage Upon receipt store kit at -20°C, or store individual components as indicated above. Kit is shipped with dry ice.
Form Liquid
Buffer Lysis Buffer
Reagent Type Cell Lysis Buffer, Detergent Solution, Subcellular Fractionation
Sufficient For 50 Samples
Quantity 1 Kit

The kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells. Extracts obtained with the Subcellular Protein Fractionation Kit for Cultured Cells are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays. Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Thermo Scientific Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.

Highlights:

  • Concise – extract functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than three hours

  • Convenient – perform a simple procedure without using ultracentrifugation over gradients

  • Complete – obtain cytoplasmic, membrane, soluble nuclear, chromatin-bound and cytoskeletal protein fractions from a single kit

  • Cultured Cells – developed and optimized for use with cultured mammalian cells; for tissue samples use the Subcellular Fractionation Kit for Tissues, Part No. 87790

  • Compatible – use with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays

  • Small-scale – kit provides sufficient reagents to fractionate 50 cell pellets of 20μL each, equivalent to a total of 2g of cell paste or tissue

Includes:

Four extraction buffers, micrococcal nuclease enzyme, calcium chloride solution and protease inhibitor cocktail

Recommended for:

Extraction and enrichment of proteins from different cellular compartments; Separation and profiling of protein from the cytoplasm, nucleus, membrane and cytoskeleton from the same cell sample; Studying protein translocation

Alternative Products
Documentation

Technical documents

Safety and Handling

[Subcellular Protein Fractionation Kit for Cultured Cells]

[Warning]

[{code=EYE DAM. 2, sequence=1, description=Serious eye damage/eye irritation Category 2}, {code=SKIN IRRIT. 2, sequence=2, description=Skin corrosion/irritation Category 2}]

[{code=H315, sequence=1, description=Causes skin irritation.}, {code=H319, sequence=2, description=Causes serious eye irritation.}]

[{code=P264, sequence=51, description=Wash thoroughly after handling.}, {code=P280, sequence=52, description=Wear protective gloves/protective clothing/eye protection/face protection.}, {code=P305+P351+P338, sequence=53, description=IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.}]

[{code=MIXTURE LIST, sequence=1, description=Contains : 4-(2-aminoethyl)-Benzenesulfonyl fluoride hydrochloride}]

[{code=GHS07, sequence=1, description=EXCLAMATION MARK}]


Product Identifier
  • Subcellular Protein Fractionation Kit for Cultured Cells

Signal Word
  • Warning

Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2

Hazard Statement
  • H315- Causes skin irritation.
  • H319- Causes serious eye irritation.

Precautionary Statement
  • P264- Wash thoroughly after handling.
  • P280- Wear protective gloves/protective clothing/eye protection/face protection.
  • P305+P351+P338- IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental Information
  • MIXTURE LIST- Contains : 4-(2-aminoethyl)-Benzenesulfonyl fluoride hydrochloride
Special Offers